9Z9E
Structure of FabS1CE2_ER-3c in complex with the extracellular region of EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979346 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.060, 72.116, 126.211 |
| Unit cell angles | 90.00, 101.65, 90.00 |
Refinement procedure
| Resolution | 43.110 - 2.400 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.054 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.112 | 2.443 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 1.240 |
| Rmeas | 0.168 | 1.619 |
| Rpim | 0.106 | 1.029 |
| Number of reflections | 66379 | 3445 |
| <I/σ(I)> | 7.9 | 1.2 |
| Completeness [%] | 95.1 | 100 |
| Redundancy | 4.3 | 4.5 |
| CC(1/2) | 0.988 | 0.364 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M potassium thiocyanate, 20% PEG3350, cryoprotected with this buffer plus 30% ethylene glycol |
