9YXD

Crystal structure of recombinant human follicle stimulating hormone in complex with an anti-FSH alpha Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	76.010, 101.103, 161.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.850 - 2.290
R-factor	0.203
Rwork	0.201
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5084)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.850	2.350
High resolution limit [Å]	2.290	2.290
Number of reflections	56196	3969
<I/σ(I)>	33.8	5.5
Completeness [%]	98.6
Redundancy	13.2
CC(1/2)	1.000	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.05 M Calcium acetate, 0.1 M Bis-Tris pH 7, 30% v/v PEG 550 MME