9YKS
Crystal structure of the G9a (EHMT2) SET domain in complex with SAM and TNG917
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.600, 76.980, 134.140 |
| Unit cell angles | 90.00, 89.80, 90.00 |
Refinement procedure
| Resolution | 45.600 - 1.790 |
| R-factor | 0.1948 |
| Rwork | 0.192 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.069 | 0.693 |
| Rpim | 0.043 | 0.432 |
| Number of reflections | 108490 | 5349 |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.999 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 33% PEG4000, 200 mM sodium acetate, 100 mM MES, pH 6.5 |
