9YE9

Structure of UbcH5b in complex with the U-box domain of the E3 ubiquitin ligase CHIP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2022-12-18
Detector	RDI CMOS_8M
Wavelength(s)	1.000020
Spacegroup name	P 21 21 21
Unit cell lengths	58.081, 59.138, 112.834
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.440 - 2.010
R-factor	0.245
Rwork	0.241
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.440	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.160
Rmeas	0.192
Rpim	0.105	0.810
Number of reflections	46470	2619
<I/σ(I)>	3.65	0.9
Completeness [%]	94.1	99.8
Redundancy	6.3	6.4
CC(1/2)	0.988	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M Magnesium chloride hexahydrate, 0.1 M Potassium chloride, 0.1 M PIPES, 20 % v/v PEG Smear Medium