9X4F
Crystal structure of tetrameric alpha-enolase with asymmetric flexible active sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.791, 115.007, 119.748 |
| Unit cell angles | 90.00, 104.23, 90.00 |
Refinement procedure
| Resolution | 42.650 - 2.700 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.985 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.940 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.136 | 0.485 |
| Number of reflections | 47895 | 4417 |
| <I/σ(I)> | 8.1 | 2.7 |
| Completeness [%] | 99.3 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291.15 | 22% PEG 8000, 0.1M Sodium cacodylate, 0.2 M Sodium acetate trihydrate |
