9WA7

Crystal Structure of Human NFIX in Complex with TGGCA(N3)TGCCA Palindromic DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	77.013, 93.341, 41.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.180 - 2.310
R-factor	0.2261
Rwork	0.224
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qqd
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmeas	0.185	0.799
Rpim	0.059	0.264
Number of reflections	13804	1323
<I/σ(I)>	120.7
Completeness [%]	99.9
Redundancy	9.9
CC(1/2)		0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.04 M Potassium Phosphate monobasic, 16% (w/v) PEG 8000, and 20% (v/v) Glycerol