9W2N

Crystal structure of the mutant LC3B in complex with p62

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2025-02-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 65
Unit cell lengths	73.991, 73.991, 40.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.240 - 1.670
R-factor	0.16904
Rwork	0.167
R-free	0.20256
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zjd
RMSD bond length	0.008
RMSD bond angle	1.824
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.730
High resolution limit [Å]	1.670	1.670
Number of reflections	14894	1367
<I/σ(I)>	30.24
Completeness [%]	99.2
Redundancy	17.7
CC(1/2)	0.970	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	2 M Ammonium sulphate, 200 mM Potassium sodium tartarate tetrahydrate, 100 mM sodium citrate pH 5.6