9VN5
Structure of WDR5 in complex with MBD3C R43K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.265, 47.376, 129.472 |
| Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
| Resolution | 43.300 - 1.900 |
| R-factor | 0.2065 |
| Rwork | 0.205 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.839 |
| Data reduction software | autoPX |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.055 | 0.436 |
| Number of reflections | 50854 | 5044 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 98.6 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.988 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M imidazole malate (pH 8.5) and 7.5% w/v PEG 10,000 |
