9VLF

Structural studies on the conformation changes induced by ligand binding in an Adenine phosphoribosyltransferase (FnAPRT) from Fusobacterium nucleatum

Replaces:  9L6J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	73.048, 106.523, 47.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 1.610
R-factor	0.1942
Rwork	0.194
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.660
High resolution limit [Å]	1.610	1.610
Number of reflections	24571	2416
<I/σ(I)>	2.91
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.996	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	pH 8.2, 1.8M NaH2PO4/K2HPO4