9VFL

Crystal structure of PLCG1 SH2(C) domain with a triple Tyr phosphorylated peptide derived from NTRK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.96183
Spacegroup name	P 41 21 2
Unit cell lengths	157.690, 157.690, 158.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.700 - 2.400
R-factor	0.2176
Rwork	0.215
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	XDS (Jun 30, 2023)
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.21.1_5286)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.700	4.160	2.550
High resolution limit [Å]	2.400	3.600	2.400
Number of reflections	78013	8155	12465
<I/σ(I)>	15.71
Completeness [%]	100.0	100	100
Redundancy	26.5
CC(1/2)	1.000	0.999	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M NaCl, 0.1 M phosphate-citrate, pH 4.2, 20% PEG8000