9RP9

Crystal structure of mouse pVHL-ElonginB-ElonginC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	C 1 2 1
Unit cell lengths	73.480, 59.780, 91.670
Unit cell angles	90.00, 100.85, 90.00

Refinement procedure

Resolution	46.040 - 2.100
R-factor	0.18447
Rwork	0.182
R-free	0.22172
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.729
Data reduction software	XDS (0.6.7.1)
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.040	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.058	0.559
Rmeas	0.082	0.791
Rpim	0.058	0.559
Number of reflections	22839	1779
<I/σ(I)>	10.5	1.3
Completeness [%]	99.5
Redundancy	1.9
CC(1/2)	0.997	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20 mM Hepes, pH 7.5, 100 mM NaCl, 1 mM DTT