9Q7W

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	108
Detector technology	PIXEL
Collection date	2017-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	39.224, 70.489, 166.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.664 - 2.537
R-factor	0.2179
Rwork	0.213
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.620
High resolution limit [Å]	2.530	5.450	2.530
Rmerge	0.103	0.080	0.362
Rmeas	0.109	0.084	0.385
Rpim	0.032	0.026	0.127
Total number of observations	183136
Number of reflections	15840	1715	1510
<I/σ(I)>	9.5
Completeness [%]	98.6	97.7	95.5
Redundancy	11.6	11.2	7.8
CC(1/2)		0.996	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	150mM calcium acetate, 100mM Tris pH 8.0, 22.55 PEG 3350