9Q1N
Crystal structure of human TEAD2-Yap binding domain covalently bound to an allosteric regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2021-04-15 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.07216 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.593, 61.467, 80.174 |
| Unit cell angles | 90.00, 117.77, 90.00 |
Refinement procedure
| Resolution | 53.150 - 2.130 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e5g |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.778 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.150 | 2.480 |
| High resolution limit [Å] | 2.130 | 2.170 |
| Rmeas | 0.134 | 0.749 |
| Rpim | 0.070 | 0.479 |
| Number of reflections | 13935 | 696 |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 90.0 | 62.1 |
| Redundancy | 3.7 | 3.3 |
| CC(1/2) | 0.995 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium Formate 1.8M - 2.4M HEPES pH 7.2 - 7.4 |
