9PLP

Crystal structure of netrin-1 in complex with anti-netrin-1 Fab (NP137 Fab)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-11-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	67.984, 263.049, 68.765
Unit cell angles	90.00, 92.51, 90.00

Refinement procedure

Resolution	49.390 - 4.990
Rwork	0.286
R-free	0.32110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	5.410
High resolution limit [Å]	4.990	4.990
Number of reflections	9577	2611
<I/σ(I)>	5.4
Completeness [%]	97.1
Redundancy	3.3
CC(1/2)	0.990	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG8000, 100 mM HEPES pH 7.5, 200 mM Ca Acetate