9PHV

Crystal structure of human KDM2A with substrate competitive inhibitor 183c

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.48310
Spacegroup name	P 31 2 1
Unit cell lengths	81.374, 81.374, 124.023
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.470 - 2.160
R-factor	0.1807
Rwork	0.179
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.691
Data reduction software	XDS (20190315)
Data scaling software	XDS (20190315)
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.470	2.290
High resolution limit [Å]	2.160	2.160
Rmeas	0.061	0.567
Number of reflections	47966	6658
<I/σ(I)>	14.38	1.28
Completeness [%]	98.4	83.5
Redundancy	4.58	2.13
CC(1/2)	0.998	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	19% PEG 2000 MME, 0.1M Tris pH 8.0, 0.2M TMANO, 8% glycerol