9PD2

Crystal structure of PILRA in complex with Fab portion of antagonist antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	37.854, 46.753, 335.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.347 - 2.579
Rwork	0.226
R-free	0.27740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.594
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.347	46.300	2.720
High resolution limit [Å]	2.579	8.160	2.580
Number of reflections	18280	722	2450
<I/σ(I)>	5.2
Completeness [%]	92.3		86.6
Redundancy	2.6
CC(1/2)	0.986	0.993	0.301

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M CAPS pH 10.5, 20% PEG 8000, 0.2 M NaCl