9OBN

Crystal structure of malaria transmission-blocking antigen Pfs48/45 C-terminal domain in complex with nanobody B2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 2 21 21
Unit cell lengths	47.690, 88.522, 281.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.540 - 2.500
R-factor	0.2107
Rwork	0.208
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.540	47.540	2.650
High resolution limit [Å]	2.500	7.420	2.500
Rmerge	0.248	0.097	1.387
Rmeas	0.261	0.101	1.475
Number of reflections	42503	1814	6759
<I/σ(I)>	8.38
Completeness [%]	99.8
Redundancy	10.5
CC(1/2)	0.993	0.996	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2 M lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 5.5, 25 %w/v PEG 3350