9NXS
Crystal structure of the post-reactive state of porcine OAS1 in complex with dsRNA and products 25A2 and PPi bound to the catalytic center.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03323 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.070, 72.070, 207.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.770 - 1.800 |
| Rwork | 0.196 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.017 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.770 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.102 | 0.885 |
| Number of reflections | 51650 | 7571 |
| <I/σ(I)> | 22.59 | 2.61 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 52.88 | 53.66 |
| CC(1/2) | 1.000 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Tris HCl pH 7.6, 33% (v/v) PEG200 |
