9NR2

Crystal structure of H5 hemagglutinin from the influenza virus A/black swan/Akita/1/2016 with LSTa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	187.241, 100.397, 132.067
Unit cell angles	90.00, 125.93, 90.00

Refinement procedure

Resolution	35.650 - 2.710
R-factor	0.2258
Rwork	0.223
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.650	2.760
High resolution limit [Å]	2.710	2.710
Rmeas	0.240	1.500
Rpim	0.100	0.630
Number of reflections	52384	5206
<I/σ(I)>	13.2
Completeness [%]	97.7
Redundancy	6.2
CC(1/2)	0.980	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium iodide, 20% PEG 3350