9NQ5

D-Ornithine/D-lysine decarboxylase C387A complexed with HEPES, putrescine, and D-ornithine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 21
Unit cell lengths	89.990, 104.770, 106.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.670 - 1.740
R-factor	0.1797
Rwork	0.179
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.670	1.760
High resolution limit [Å]	1.740	1.740
Number of reflections	103202	6405
<I/σ(I)>	7.22	1.16
Completeness [%]	99.8
Redundancy	6.3
CC(1/2)	0.998	0.261

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES, pH 7.5, 0.2-0.4 M NaOAc, 20-24% PEG 3350