9LTP
Crystal structure of human RAB43 in GDP-AlF3 transition state complex with USP6NL TBC domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-23 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979098 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.669, 65.483, 98.760 |
| Unit cell angles | 89.40, 76.73, 67.36 |
Refinement procedure
| Resolution | 35.990 - 2.490 |
| R-factor | 0.2421 |
| Rwork | 0.239 |
| R-free | 0.29820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.428 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Rmerge | 0.140 | 0.373 |
| Number of reflections | 37977 | 3714 |
| <I/σ(I)> | 7.2 | 3.1 |
| Completeness [%] | 95.8 | 94.4 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.984 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Bis-Tris propane, pH8.5; 20% PEG 3350; 0.02M Na/K phosphate pH7.4 |
