9LSS

The crystal structure of PDE5 with 1934

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-06
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	73.750, 73.750, 128.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.237 - 2.500
R-factor	0.216232731989
Rwork	0.214
R-free	0.27107
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h42
RMSD bond length	0.009
RMSD bond angle	1.012
Data reduction software	CrysalisPro (v2)
Refinement software	REFMAC (V1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.240	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.088	0.867
Number of reflections	14545	1433
<I/σ(I)>	19.7	2.1
Completeness [%]	100.0	100
Redundancy	8.5	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M MgSO4, 0.1 M Cacodylate sodium salt, pH 6.5, 2.5% ethanol, and 18% PEG3350