9LSL

The crystal structure of PDE5A with L1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-30
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.54184
Spacegroup name	P 31 2 1
Unit cell lengths	74.419, 74.419, 132.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.440 - 2.500
R-factor	0.2457
Rwork	0.243
R-free	0.29610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.509
Data reduction software	CrysalisPro (V2.0)
Refinement software	REFMAC (V1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.440	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.130	0.580
Number of reflections	15180	100
<I/σ(I)>	13.6	3.4
Completeness [%]	100.0
Redundancy	9.2	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M MgSO4, 0.1 M Cacodylate sodium salt, pH 6.5, 2.5% ethanol, and 18% PEG3350