9LSF
Crystal structure of mRFP1 with a grafted calcium-binding sequence and one bound calcium ion in a calcium-free solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.250, 86.010, 109.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.080 - 1.620 |
| R-factor | 0.1759 |
| Rwork | 0.175 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.080 | 1.720 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 68395 | 10934 |
| <I/σ(I)> | 23.27 | |
| Completeness [%] | 99.7 | |
| Redundancy | 10.6 | |
| CC(1/2) | 1.000 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 0.1M MgCl2, 25% (w/v) PEG 400, 20% (w/v) PEG 3350 |
