9KTK

Crystal structure of human SIRT3 with its activator SKLB-11A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	71.582, 120.362, 134.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.470 - 2.490
R-factor	0.2655
Rwork	0.262
R-free	0.34020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	7.759
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.490	2.630
High resolution limit [Å]	2.490	2.490
Number of reflections	41592	3187
<I/σ(I)>	18.7
Completeness [%]	99.9
Redundancy	12.6
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Li2SO4, 1.1M (NH4)2SO4, 0.1M Tris-HCl pH 7.5