9IM4

Crystal Structure of AF9 YEATS domain F28R mutant in complex with histone H3K9la

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	83.081, 83.081, 116.259
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.591 - 2.794
R-factor	0.2711
Rwork	0.266
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.093
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.790	7.590	2.800
Rmerge	0.131	0.057	0.516
Rmeas	0.137	0.060	0.543
Rpim	0.039	0.020	0.162
Number of reflections	11943	657	580
<I/σ(I)>	6.6
Completeness [%]	99.7	98.1	99
Redundancy	11.2	9.2	8.1
CC(1/2)	0.989	0.998	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2.1M DL Malic acid pH7.0