9IKQ

Crystal structure of OPTN LZD in complex with GTP-bound Rab8a(Q67L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98655
Spacegroup name	P 21 21 21
Unit cell lengths	67.218, 69.432, 110.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.190 - 1.930
R-factor	0.1763
Rwork	0.174
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.400	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.131	0.899
Number of reflections	39526	1833
<I/σ(I)>	13.5
Completeness [%]	99.8
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1 M BIS-TRIS pH 6.5, 16% (w/v) PEG 10000