9I1Q
HER3 receptor in complex with the Fab fragment of TK-hu A3 monoclonal antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.030, 98.810, 270.380 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.800 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.760 | 2.940 |
| High resolution limit [Å] | 2.550 | 2.551 |
| Rmerge | 0.120 | 1.029 |
| Number of reflections | 82868 | 4143 |
| <I/σ(I)> | 12.3 | 1.9 |
| Completeness [%] | 87.6 | 63.7 |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.999 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M KSCN, 0.1 M BIS-TRIS propane pH 8.5, 14% PEG 3350 |
