Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.461, 67.043, 101.845 |
| Unit cell angles | 90.00, 94.06, 90.00 |
Refinement procedure
| Resolution | 46.780 - 2.500 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 2.690 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 1.147 |
| Rmeas | 0.087 | 1.238 |
| Rpim | 0.034 | 0.463 |
| Number of reflections | 15196 | 2987 |
| <I/σ(I)> | 10.3 | 1.37 |
| Completeness [%] | 93.8 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.7 | 277 | 9-11% PEG 8000, 0.2 M magnesium acetate and 0.1 M sodium cacodylate, pH 5.7 |
