9GVO

type-I interferons autoantibodies pmab15 and pmab14 in complex with Interferon alpha-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 31 2 1
Unit cell lengths	112.930, 112.930, 155.974
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.990 - 1.810
R-factor	0.1936
Rwork	0.193
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.940
Data reduction software	xia2 (3.8.6)
Data scaling software	xia2 (3.8.6)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.146	5.184
Rmeas	0.147	5.313
Rpim	0.019	1.159
Number of reflections	105167	5103
<I/σ(I)>	17.1	0.5
Completeness [%]	100.0
Redundancy	55.7
CC(1/2)	1.000	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Hepes pH 7.5 0.8 M Na2H2PO4 0.8 M KH2PO4