9GP1

Jumonji domain-containing protein 2A with crystallization epitope mutatios K330R:A334E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	57.030, 101.600, 141.430
Unit cell angles	90.00, 99.38, 90.00

Refinement procedure

Resolution	82.135 - 2.210
Rwork	0.215
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.757
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.82))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.600	2.270
High resolution limit [Å]	2.210	2.210
Number of reflections	77812	5675
<I/σ(I)>	11.7
Completeness [%]	97.8
Redundancy	3.4
CC(1/2)	0.993	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M ammonium acetate, 25% PEG3350, 0.1M bis-tris pH 6.5