9GOC

Crystal structure of DPP9 Ser730Ala in complex with sulphostin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99998
Spacegroup name	P 1
Unit cell lengths	88.613, 106.531, 121.270
Unit cell angles	65.15, 70.72, 76.35

Refinement procedure

Resolution	83.240 - 1.890
R-factor	0.17691
Rwork	0.177
R-free	0.19945
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.321
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.240	1.930
High resolution limit [Å]	1.890	1.890
Number of reflections	288843	14452
<I/σ(I)>	6
Completeness [%]	96.2
Redundancy	2.2
CC(1/2)	0.990	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG2000 MME 20% (w/v), 0.1 M Tris pH 6.75