9EY5
Crystal structure of human tyrosinase-related protein 1 (TYRP1) in complex with (S)-2,4-dihydroxyphenylalanine
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8856, 1.2782, 1.7220 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.152, 103.152, 138.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.330 - 2.610 |
| R-factor | 0.1982 |
| Rwork | 0.196 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.330 | 2.700 |
| High resolution limit [Å] | 2.607 | 2.610 |
| Rmerge | 0.141 | 0.696 |
| Rmeas | 0.164 | 0.808 |
| Rpim | 0.081 | 0.401 |
| Number of reflections | 47641 | 4767 |
| <I/σ(I)> | 4.94 | 1.01 |
| Completeness [%] | 98.6 | 98.33 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.988 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 6000, Zinc chloride, Tris-HCl pH8.0 |
