9EXX

Crystal structure of the PWWP1 domain of NSD2 bound by compound 18.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	50.149, 54.939, 149.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.810 - 1.943
R-factor	0.2344
Rwork	0.233
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.812	2.131
High resolution limit [Å]	1.943	1.943
Number of reflections	21582	1081
<I/σ(I)>	17.6
Completeness [%]	93.1
Redundancy	6.6
CC(1/2)	0.999	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30 % ethanol