9EBW

Chimeric fluorescence biosensor formed from a lactate-binding protein and GFP, bound to lactate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920119
Spacegroup name	P 1 21 1
Unit cell lengths	90.878, 109.803, 123.711
Unit cell angles	90.00, 110.14, 90.00

Refinement procedure

Resolution	45.940 - 2.780
R-factor	0.2437
Rwork	0.242
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.356
Data reduction software	XDS (1.19)
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	2.880
High resolution limit [Å]	2.780	2.780
Rmerge	0.148	0.689
Rmeas		0.775
Rpim	0.070	0.345
Number of reflections	55034	5330
<I/σ(I)>	7.4	1.82
Completeness [%]	95.4
Redundancy	4.6
CC(1/2)	0.992	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	citric acid, PEG 6000, 1,3-propanediol