9E8P

Crystal Structure of GABARAP-ATG3 conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	97.388, 97.388, 166.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.330 - 2.700
R-factor	0.2205
Rwork	0.218
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.330	2.796
High resolution limit [Å]	2.700	2.700
Rmerge	0.060	1.232
Rpim	0.020	0.408
Number of reflections	41972	4400
<I/σ(I)>	24.97	1.95
Completeness [%]	99.6	99.46
Redundancy	10.1	9.8
CC(1/2)	1.000	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	0.1 mM Tris pH8.5, 25%(w/v) PEG3550