9E7K

Crystal structure of the UCH37 RPN13 DEUBAD complex bound to an inhibitory nanobody in the canonical ubiquitin binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	178.634, 190.732, 45.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.310 - 2.410
R-factor	0.2363
Rwork	0.235
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.584
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.310	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.066	0.831
Rmeas	0.079	0.978
Rpim	0.043	0.508
Number of reflections	29105	2823
<I/σ(I)>	14.72
Completeness [%]	94.5
Redundancy	3.4
CC(1/2)	0.998	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	0.1 M Sodium citrate tribasic dihydrate pH 5.6, 2% v/v Tacsimate pH 5.0, 16% w/v Polyethylene glycol 3,350