9E6Y

Structure of CD112 (Nectin-2) domain 1 bound to CD112R (PVRIG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	79.650, 79.650, 81.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.240 - 2.200
R-factor	0.2096
Rwork	0.208
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.240	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	13826	1362
<I/σ(I)>	17.6
Completeness [%]	99.9
Redundancy	19.57
CC(1/2)	0.999	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	298	0.1M CHES PH 9.5, 20% W/V PEG 8000