9E4V

The structure of human vacuolar protein sorting 34 catalytic domain bound to MES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979460
Spacegroup name	P 41 21 2
Unit cell lengths	114.088, 114.088, 145.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.310 - 2.360
R-factor	0.2002
Rwork	0.199
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASES
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.310	2.444
High resolution limit [Å]	2.360	2.360
Rmerge	0.205	3.989
Rmeas	0.209	4.067
Rpim	0.040	0.786
Number of reflections	40128	3953
<I/σ(I)>	15.27	1.13
Completeness [%]	100.0	99.95
Redundancy	26.9	26.4
CC(1/2)	0.999	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	100mM MES, pH 6.0, 18% PEG3350