9E2T

Structure of a de novo designed interleukin-21 mimetic complex with IL-21R and IL-2Rg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 1
Unit cell lengths	75.499, 78.082, 101.219
Unit cell angles	94.63, 103.39, 114.55

Refinement procedure

Resolution	41.170 - 2.280
R-factor	0.2006
Rwork	0.199
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.238	2.544
High resolution limit [Å]	2.280	2.280
Number of reflections	84630	2417
<I/σ(I)>	5.17
Completeness [%]	92.4
Redundancy	5.6642
CC(1/2)	0.996	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.2M Potassium thiocyanate, 0.1M Bis-Tris propane, pH 6.5, 20% w/v PEG 3350