9CYL
Structure of LAG3 loop1 deletion bound to the MHC class II molecule I-A(b)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-03-12 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.97856 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 184.994, 184.994, 122.672 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 92.500 - 4.660 |
R-factor | 0.2709 |
Rwork | 0.268 |
R-free | 0.32570 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.569 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.500 | 13.860 | 4.940 |
High resolution limit [Å] | 4.660 | 9.850 | 4.660 |
Rmerge | 0.187 | 0.045 | 2.570 |
Rmeas | 0.198 | ||
Number of reflections | 7006 | 512 | 1098 |
<I/σ(I)> | 10.91 | 46.49 | 1.13 |
Completeness [%] | 99.8 | 100 | 99.4 |
Redundancy | 10.5 | 10.37 | 11.2 |
CC(1/2) | 0.992 | 0.999 | 0.387 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293.15 | 1% w/v Tryptone, 0.001 M Sodium azide, 0.05 M HEPES sodium pH 7.0, 12% w/v Polyethylene glycol 3,350 |