9CPL

The ubiquitin-associated domain of human thirty-eight negative kinase 1, fused to the 2TEL crystallization chaperone via a 2-glycine linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 65
Unit cell lengths	105.725, 105.725, 50.917
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.780 - 2.400
R-factor	0.2313
Rwork	0.229
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.480
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.780	2.400
High resolution limit [Å]	2.400	2.400
Rmerge	0.103	3.597
Rmeas	0.108	3.786
Rpim	0.034	1.173
Number of reflections	12862	1150
<I/σ(I)>	10.88	0.62
Completeness [%]	99.1	92
Redundancy	10.2	10.2
CC(1/2)	0.999	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	100mM BisTris, pH 6.0, 960mM Mg-formate