9CP0

Crystal structure of the porcine astrovirus 4 capsid spike domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	46.386, 66.211, 87.752
Unit cell angles	74.54, 81.67, 78.61

Refinement procedure

Resolution	39.780 - 1.850
R-factor	0.3175
Rwork	0.316
R-free	0.36170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.743
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.780	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	80526	3920
<I/σ(I)>	8.1	2.1
Completeness [%]	96.1
Redundancy	3.2
CC(1/2)	0.984	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.2 M calcium acetate hydrate, 0.1 M Tris-Cl pH 7, and 12% PEG 3000