9CBS
Crystal structure of Chaetomium thermophilum Gcn2 HisRS-like domain, catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.091, 132.554, 138.961 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.490 - 2.690 |
| R-factor | 0.1779 |
| Rwork | 0.177 |
| R-free | 0.20960 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.210 | 1.270 |
| Rpim | 0.037 | 0.283 |
| Number of reflections | 65096 | 3228 |
| <I/σ(I)> | 48.1 | 4 |
| Completeness [%] | 98.8 | 93.8 |
| Redundancy | 33.2 | 20 |
| CC(1/2) | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.15 M MgCl2, 0.15 M CaCl2, 100 mM HEPES pH 7.5, 20% v/v PEG 500 MME, and 5% PEG 20000 |
