9BZ4
Crystal structure of the C2 and GAP domains of human p120RasGAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.894, 94.036, 96.713 |
| Unit cell angles | 95.81, 111.10, 108.51 |
Refinement procedure
| Resolution | 87.520 - 2.450 |
| R-factor | 0.2309 |
| Rwork | 0.228 |
| R-free | 0.28360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.562 |
| Data reduction software | XDS (20220110) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.520 | 2.570 |
| High resolution limit [Å] | 2.440 | 2.450 |
| Rmerge | 0.308 | 1.495 |
| Rmeas | 0.340 | 1.728 |
| Rpim | 0.108 | 0.641 |
| Number of reflections | 75972 | 10823 |
| <I/σ(I)> | 12.1 | 1.7 |
| Completeness [%] | 96.3 | 93.9 |
| Redundancy | 10 | 7 |
| CC(1/2) | 0.989 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.2M Ammonium Phosphate Dibasic 0.1M MOPS pH 7.4 26% w/v PEG 3350 |
