9BVT

RNA Pol II - High Mn(+2) concentration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-21
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	221.190, 393.970, 283.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.250 - 3.400
R-factor	0.2215
Rwork	0.220
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	197.000	3.650
High resolution limit [Å]	3.350	3.350
Number of reflections	175204	10487
<I/σ(I)>	6.2
Completeness [%]	99.0
Redundancy	4.5
CC(1/2)	0.920	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Tacsimate Hepes