9BOL
Crystal structure of the complex between VHL, ElonginB, ElonginC, and compound 5
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.460, 47.740, 100.350 |
| Unit cell angles | 103.62, 94.66, 98.33 |
Refinement procedure
| Resolution | 46.660 - 1.990 |
| R-factor | 0.20394 |
| Rwork | 0.202 |
| R-free | 0.24791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.048 | 0.530 |
| Number of reflections | 52695 | 3923 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 91.4 | |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 mM sodium cacodylate pH 5.7, 15% PEG8000, 0.2 M magnesium acetate, 5 mM DTT |
