9BFB
Crystal structure of BRAF kinase domain with PF-07284890
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-11 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.609, 97.609, 69.311 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.020 - 1.920 |
| R-factor | 0.1656 |
| Rwork | 0.164 |
| R-free | 0.18880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.937 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.020 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.077 | 0.566 |
| Rmeas | 0.081 | 0.609 |
| Rpim | 0.027 | 0.220 |
| Number of reflections | 29350 | 1965 |
| <I/σ(I)> | 17.6 | 3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 9 | 7.4 |
| CC(1/2) | 0.999 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 100mM Hepes pH 7.0, 15 % PEG 8K, 10% Tacsimate |
