9BD3

Structure of the MAGEA4 MHD-RAD18 R6BD Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 65
Unit cell lengths	85.210, 85.210, 206.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.600 - 2.580
R-factor	0.1929
Rwork	0.191
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.600	2.672
High resolution limit [Å]	2.580	2.580
Rmerge	0.065	0.818
Rpim	0.028	0.361
Number of reflections	26114	2606
<I/σ(I)>	19.04	3.79
Completeness [%]	98.2
Redundancy	6.1
CC(1/2)	0.999	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% (w/v) PEG 8000 100 mM HEPES/ Sodium hydroxide, pH 7.5, 200 mM Ammonium sulfate, 10% (v/v) 2-propanol