9BCZ

Chicken 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) in complex with calcium and phosphorylated threonine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95307
Spacegroup name	C 1 2 1
Unit cell lengths	122.182, 85.539, 98.433
Unit cell angles	90.00, 112.04, 90.00

Refinement procedure

Resolution	49.590 - 1.990
R-factor	0.17439
Rwork	0.173
R-free	0.20412
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.581
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.990	5.430	2.000
Rmerge	0.163	0.058	0.927
Rmeas	0.178	0.063	1.031
Rpim	0.070	0.024	0.443
Number of reflections	60478	3276	2409
<I/σ(I)>	6.4
Completeness [%]	94.7	99.8	75.8
Redundancy	6	6.7	4.3
CC(1/2)	0.989	0.997	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
265735